This website uses cookies to ensure you have the best experience. Learn more

The Harmful Influence Of Quantum Dots On Cytoskeleton Meshwork

359 words - 2 pages

Research Proposal

The Harmful Influence of Quantum Dots on Cytoskeleton Meshwork

Recent study suggests that Quantum Dot exposure causes significant morphological changes to macrophages; cells became round and condensed with fewer surface protrusions in response to QD treatment, it has also been demonstrated that QD’s intracellular localization undermined the capability of macrophagic phagocytosis.

Since actin is fundamental to cellular morphological changes, it would be worthwhile to investigate the activity between QDs and the filamentous ...view middle of the document...

I will also be looking

Confocal Microscopy
The nuclei will be stained with 4',6-diamidino-2-phenylindole (DAPI, blue) and the cytoskeleton with FITC-conjugated phalloidin (green). The fluorescence for nuclei, cytoskeleton and QDs will be assessed through confocal laser scanning microscopy. All three types of cells will be examined by confocal microscopy. Through this experiment, I will be observing the relative position of QDs in the cell, and the reduction in the density of actin meshwork and in the numbers of actin rich surface protrusion in cells upon exposure to QDs.

Protein Pulldown and Western Blot
I will first expose the cells with QDs for 24 hours, and then extract the cell protein. After extraction, most of the proteins will be in the solvent, and can be separated from QDs, cell membranes, DNA etc. by centrifugation. By Western Blotting the solvent, I will be able to see whether actin has decreased, which means that it could have attached to QDs. The sediments will be boiled and the upper solvents of the boiled material will be used in Western Blotting to see if there is actually actin in the sediment, which will further prove that actin and QDs are able to attach.

Other Papers Like The Harmful Influence Of Quantum Dots On Cytoskeleton Meshwork

On The Effects Of Globalization Essay

382 words - 2 pages joined the workforce, expansion of higher education, fewer babies. 1990 Less and less newborns, very small compared to middle aged and old. Moving forward with projection, it's extremely top heavily. Welfare is projected to be one working person for every person on welfare. Rapidly aging population, although China's is aging faster. China will have 300 million people over 60. Aging population coupled with shrinking economy. Less

The Effects Of Media On Body Image

2449 words - 10 pages battled with anorexia until it ended her life at the young age of twenty seven. In modern culture, a great deal of importance is placed on our looks and body image. This is portrayed by the media through magazine pictures, television advertisements, billboards, and the influence of models and actresses. Although the media affects both men and women, I will be showing how it specifically affects the behaviors, viewpoints, and attitudes of women

Impacts Of Human Behavior On The Environment

1213 words - 5 pages RUNNING HEAD: Impacts of Human Behavior on the Environment 1 Impacts of Human Behavior on the Environment Psy 460 Impacts of Human Behavior on the Environment 2 Environmental psychology is the study of the link between human behavior, the direct impact on the environment, and the psychology of humans. The physical or manmade environment such as

Socrates On The Equality Of Women

1314 words - 6 pages speaking in regards to knowledge and the different parts of the soul i.e ( reason, appetite, spirit). Here Socrates holds that individual should be judjed based on the virtue of their souls, and not mere physical appearance . a mans virtue is not brought about by his sex, virtue remains virtue regardless of a mans gender, socrates holds this postion due to this the best state must provide women the opportunity to govern. Socrates holds that role

The View Of College Students On Love

2055 words - 9 pages The View of College Students on Love Abstract Love has been the noticeable problem of college students in modern times. The sense of loneliness and freedom of living in different places lead the college students to fall in love in an earlier age. These years, with the invasion of western sexual liberalism, our sense of morality has been faced with big challenges. At the same time, free access to the Internet causes misunderstanding and

Reflection On The Purpose Of A Portfolio

809 words - 4 pages its fullest potential, a portfolio serves as a repository for documents, certificates and other related artefacts, a workspace for reflecting on professional competence and evaluating learning needs, and as a showcase for presenting evidence that demonstrates current competence and future abilities across a range of criteria in a variety of contexts (Andre & Heartfield, 2007). The structure and content of a portfolio can vary and is dependent on

The Effect Of The Media On Aggression In Children

1117 words - 5 pages The Effect of the Media on Aggression in Children. Media is of importance to many people for many reasons, including entertainment and escapism. The mass production of televisions in the 1950’s has led to a phenomenon previously unheard of, with statistics showing that as of 2013, 79% of the world has at least one television set per household (TVTechnology, 2013). It therefore stands to reason that children may have easy access to media that

An Essay On The Original Intent Of The Second Amendment

2488 words - 10 pages individual right. It was in 1934 that the first attempt at universal gun control on a national level occurred. In 1934, the United States was at the height of the Great Depression (Kangas, 1997). In 1933, the 18th Amendment to the Constitution had finally been repealed, marking the end of the noble experiment known as “prohibition”. The fourteen years of prohibition had nurtured an atmosphere of speakeasies, bootlegging, gangsters, and mafia. The

The Impact Of The Iom Report On Nursing

1146 words - 5 pages The Impact of the IOM Report on Nursing Lori VanDerveer-Hand Grand Canyon University October 6, 2013 The Impact of the IOM Report on Nursing Key Message #1:“Nurses should practice to the full extent of their education and training” (Institute of Medicine of the National Academies, 2010, p. 85). The impact that the IOM report has had on nursing and it’s future, as we know it has been

The Functionalist View On The Role Of Education

1302 words - 6 pages outlooks on the role of education as they both believe that it prepares us for later life and the workforce. However, some sociologist would argue that Durkheim is wrong because we all have different cultures and histories so therefore, history doesn’t bind us together because the education system is ethnocentric and also we’re not bonded together because we’re segregated by classes due to capitalism. Moreover, because of capitalism in society we do

The Impact Of Mass Media On Female Teenagers

2335 words - 10 pages The Harmful Effects of Media on Teenage Girls’ Self Image Media is one of the most effective tools in the world. People learn more from the media than any other single source of information. No matter where people look, they are constantly assaulted with images and ads, whether it is online, watching television, or even walking down the street. Because of the media’s ease of accessibility, it is vital that America understands the subliminal

Related Essays

Influence Of Mathematics On Economics Essay

302 words - 2 pages Influence of Math on Economics In: Business and Management Influence of Math on Economics Influence of Math on Economics Michelle Balmer, Marcie Holland, Beverly Segars, Israel Figueroa, and Porshia Cross MTH 110 Rigoberto Martinez March 17, 2012 The Influence of Math on Economics The history of mathematics is an investigative study of the discoveries of

Evaluate The Influence Of Two Major Theories Of Ageing On Health And Social Care Provision

1147 words - 5 pages . Conclusion In conclusion the two theories of aging have influenced health and social care provisons by showing them what older adults tend to go through once they reach the older stages of their lives. To prevent disengament health and social care services have provided clubs and opportunities for people to socialize and remain mentally active

Harnessing The Science Of Influence Essay

1045 words - 5 pages power and influence, we should pay attention on these criteria: effectiveness for the individual, effectiveness for organization, and ethical. Organizations are political entities, because it consists of interdependent individuals (and group) with divergent interests. There are challenges in Managerial Life, such as: * Interdependency: Managers are dependant on others to get things done, and the other way around. * Diversity: Differences

The Influence Of Health Care In Nursing

1452 words - 6 pages . It is important for the CH nurse to understand cultural diversity (race or ethnic) of the community. Race or ethnic origin has a limited effect on the health care status of individuals. There are diseases with a direct genetics link to race or ethnicity. (E.g. sickle cell anemia for African Americans or Tay Sachs disease for the Jewish. Beyond such clear links, the heath care status of most individuals is influence by a much broader spectrum of